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مقاله
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Abstract
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Title:
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Effects of fibrin glue as a three-dimensional scaffold in cultivated adult human retinal pigment epithelial cells.
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Author(s):
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Balagholi S1, Rezaei Kanavi M1, Alizadeh S2, Dabbaghi R3, Karami S2, Kheiri B4, Daftarian N4.
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Presentation Type:
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Poster
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Subject:
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Retina and Retinal Cell Biology
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Others:
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Presenting Author:
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Name:
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Sahar Balagholi
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Affiliation :(optional)
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1 1 Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2 2 Department of Hematology, School of Allied Medicine, Tehran University of Medical Scienc
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E mail:
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sahar_balagholi@yahoo.com
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Phone:
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Mobile:
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09367124753
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Purpose:
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This study was conducted to examine morphological, genotypic, and phenotypic alterations occurring in cultured adult human retinal pigment epithelial cells when encapsulated with different concentrations of fibrin glue
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Methods:
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Cultivated adult human retinal pigment epithelial cells were encapsulated with different concentrations of fibrin glue, namely FG1 (42 mg/dl), FG2 (84 mg/dl), FG3 (124 mg/dl), FG4 (210 mg/dl), followed by the evaluation of genetic and cytomorphological changes and protein expression.
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Results:
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Cultured adult human retinal pigment epithelial cells showed dendritiform morphology during the early days of encapsulation with fibrin glue. Moreover, an increasing inhibitory effect on cell growth was observed with increasing concentrations of fibrin glue. At the transcriptional level, the expression of MMP2, PAX6, and ITGB1 in FG1-encapsulated cells was significantly higher than that in other treated groups; however, the expression of ACTA2 was lower in all fibrin glue-encapsulated groups compared to that in the controls. Immunocytochemistry showed that FG2-encapsulated cells expressed cytokeratin 8/18, RPE65, and ZO-1 proteins, but not PAX6. In conclusion, fibrin glue at a concentration of 84?mg/dl allows proper encapsulation of adult human retinal pigment epithelial cells, while preserving the morphometric, genotypic, and phenotypic features of the cells.
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Conclusion:
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This three-dimensional biopolymer can be considered a reliable vehicle for retinal pigment epithelium cell transplantation in cell-based therapies.
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Attachment:
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13iravo poster sahar 2019.pptx
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