By default that human crystalline lens capsules (LCs) could qualify as a scaffold and intended to portray the properties of this material for endothelial tissue building.

: LCs were confined from donor's eyes, put away at −80 °C, and decellularized with trypsin‐EDTA and water. The decellularization was examined by nuclear recoloring and checking and the capsule thickness was dictated by OCT and contrasted and Descemet's film (DM). Transparency was inspected by spectrometry, and collagenase debasement was accomplished to assess its protection from corruption. Cell‐scaffold communication was surveyed by estimating central bonds surface zone on LC and plastic. At last, essential corneal endothelial cells were developed on LCs to approve the phenotype.

Trypsin‐EDTA decellularized most successfully, evacuating 99% of cells. The mean LC thickness was 34.66 ± 0.33 μm, while DM estimated 23.83 ± 0.36 μm. Light transmission was 89% for both DM and LC. On a collagenase test, LC and amniotic film were processed after 12 hr, while DM was processed after 16 hr. The surface region of central attachments for cells developed on covered LCs was something like twofold that contrasted and different conditions, while tight junctions, hexagonal morphology, and ion pumps were very much kept up when endothelial cells were cultured on LCs

LCs show very good scaffolding properties for tissue building and support the sustain the cell phenotype and can be viewed as a reasonable substrate for eye tissue designing or as a layout for future scaffolds.